| 1 | aktsiaselts Olerex | 551.60 M € | 665 |
| 2 | sihtasutus Tartu Ülikooli Kliinikum | 331.91 M € | 4136 |
| 3 | Foxway OÜ | 255.34 M € | 598 |
| 4 | AS Terminal | 192.29 M € | 245 |
| 5 | GPV Estonia AS | 178.06 M € | 749 |
| 6 | Cronimet Nordic OÜ | 144.47 M € | — |
| 7 | Osaühing Mapri Ehitus | 125.85 M € | — |
| 8 | Tulundusühistu Tartu Tarbijate Kooperatiiv | 117.07 M € | — |
| 9 | Põllumeeste ühistu KEVILI | 116.97 M € | 51 |
| 10 | aktsiaselts A. Le Coq | 107.89 M € | 378 |
| 11 | Sihtasutus Eesti Teadusagentuur | 104.70 M € | 75 |
| 12 | AS DECORA | 76.37 M € | 280 |
| 13 | GRK Eesti AS | 72.15 M € | 96 |
| 14 | AS HANZA Mechanics Tartu | 71.81 M € | 500 |
| 15 | Osaühing Eesti Viljasalv | 66.35 M € | — |
| 16 | AS Palmako | 66.30 M € | — |
| 17 | Warmeston OÜ | 64.88 M € | — |
| 18 | Tartu Mill AS | 64.36 M € | 62 |
| 19 | OÜ Ehitustrust | 63.68 M € | — |
| 20 | aktsiaselts RAIT | 59.80 M € | 151 |
| 21 | Saint-Gobain Glass Estonia SE | 59.23 M € | 316 |
| 22 | Orien Trade OÜ | 53.87 M € | — |
| 23 | osaühing Playtech Estonia | 52.99 M € | 658 |
| 24 | Gren Tartu AS | 51.77 M € | 65 |
| 25 | Embach Ehitus OÜ | 51.47 M € | 55 |
| 26 | aktsiaselts EHITUSFIRMA RAND JA TUULBERG | 51.12 M € | — |
| 27 | Elva Tarbijate Ühistu | 49.29 M € | 212 |
| 28 | Karl Bilder OÜ | 48.58 M € | 77 |
| 29 | AS GoBus | 48.52 M € | 667 |
| 30 | osaühing NÕO LIHATÖÖSTUS | 46.57 M € | 152 |
| 31 | AS Aasta Auto | 46.57 M € | 58 |
| 32 | aktsiaselts ESTIKO - PLASTAR | 45.45 M € | — |
| 33 | Kodumaja AS | 44.50 M € | — |
| 34 | osaühing ESTOVER PIIMATÖÖSTUS | 40.35 M € | 164 |
| 35 | aktsiaselts "TATOLI" | 38.13 M € | 56 |
| 36 | OÜ Vara Saeveski | 35.11 M € | 72 |
| 37 | OÜ AGRILAND | 34.56 M € | 38 |
| 38 | Henkel Balti OÜ | 32.28 M € | 49 |
| 39 | OÜ Lasita Maja | 31.75 M € | — |
| 40 | Mayeri Industries AS | 31.30 M € | — |
| 41 | aktsiaselts TARTU EHITUS | 29.91 M € | — |
| 42 | osaühing Tarmetec | 29.03 M € | 209 |
| 43 | Promens Rõngu AS | 28.89 M € | 210 |
| 44 | aktsiaselts KE INFRA | 28.54 M € | 71 |
| 45 | OÜ Tallinna Ehitustrust | 27.34 M € | 28 |
| 46 | OÜ Recar Group | 25.76 M € | 2 |
| 47 | KRC Ehitus OÜ | 25.42 M € | — |
| 48 | AS A&P Mets | 25.20 M € | — |
| 49 | Tulundusühistu Piimalaev | 24.60 M € | 2 |
| 50 | Second Round OÜ | 24.17 M € | 7 |
| 51 | Sihtasutus Tartu Kiirabi | 23.98 M € | 408 |
| 52 | Aktsiaselts SEBE | 23.48 M € | 322 |
| 53 | aktsiaselts SALVEST | 23.06 M € | 173 |
| 54 | Euroopa Transpordi OÜ | 22.35 M € | 7 |
| 55 | Balti Vara Ehitus OÜ | 21.35 M € | — |
| 56 | osaühing SAVEKATE | 21.22 M € | 63 |
| 57 | AS Pinest | 21.07 M € | 69 |
| 58 | aktsiaselts RESPO HAAGISED | 20.93 M € | — |
| 59 | aktsiaselts Tiksoja Puidugrupp | 20.91 M € | 128 |
| 60 | Tarmeko Spoon OÜ | 20.42 M € | 69 |
| 61 | Osaühing Mark Oil | 20.41 M € | 8 |
| 62 | MTÜ Eesti Pagulasabi | 19.69 M € | 129 |
| 63 | NORDIC DIGITAL AS | 19.41 M € | 81 |
| 64 | Lemeks Timber Trading OÜ | 19.30 M € | 0 |
| 65 | AS Go Oil | 19.15 M € | 7 |
| 66 | osaühing TARTU UUSAPTEEK | 18.93 M € | 21 |
| 67 | Diotech OÜ | 18.64 M € | — |
| 68 | Nordista OÜ | 18.61 M € | 20 |
| 69 | aktsiaselts TREF | 18.21 M € | — |
| 70 | Rush Street Interactive Development Estonia OÜ | 17.96 M € | — |
| 71 | aktsiaselts VALLIKRAAVI KINNISVARA | 17.78 M € | — |
| 72 | NASON DAVIS EESTI OÜ | 17.74 M € | — |
| 73 | OÜ Balt-Hellin | 17.61 M € | 31 |
| 74 | AS Aasta Auto Pluss | 17.58 M € | 32 |
| 75 | Alonafish OÜ | 17.50 M € | 3 |
| 76 | APChemicals OÜ | 17.26 M € | 35 |
| 77 | Forestex Tartu OÜ | 17.08 M € | 5 |
| 78 | aktsiaselts MAXILLA | 16.35 M € | — |
| 79 | Torm Metall OÜ | 15.77 M € | 167 |
| 80 | Inmedias Res OÜ | 15.72 M € | — |
| 81 | Liistuvabrik OÜ | 15.31 M € | 108 |
| 82 | Eesti Metsameister OÜ | 15.27 M € | — |
| 83 | Baltyre Eesti AS | 15.17 M € | — |
| 84 | Dorpat Ehitus OÜ | 15.14 M € | 20 |
| 85 | Iriscorp Transport OÜ | 15.10 M € | 52 |
| 86 | aktsiaselts METAAN | 14.92 M € | 30 |
| 87 | aktsiaselts Tartu Veevärk | 14.88 M € | 90 |
| 88 | OÜ Micotrade | 14.84 M € | 1 |
| 89 | AS KROONPRESS | 14.82 M € | 121 |
| 90 | Osaühing Data Print | 14.81 M € | 69 |
| 91 | HansaPeat OÜ | 14.78 M € | 24 |
| 92 | Aktsiaselts Topauto Tartu | 14.76 M € | 30 |
| 93 | HUUM OÜ | 14.70 M € | 36 |
| 94 | Concise Systems OÜ | 14.45 M € | 126 |
| 95 | Sihtasutus Teater Vanemuine | 14.26 M € | 329 |
| 96 | SaleMove OÜ | 14.23 M € | 137 |
| 97 | Lõuna-Eesti Hooldekeskus AS | 14.14 M € | 310 |
| 98 | Truck Trading Estonia OÜ | 14.00 M € | 55 |
| 99 | INHUS Prefab OÜ | 13.89 M € | 142 |
| 100 | ARENS AS | 13.64 M € | 84 |